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Samiei, Mohammad and Dalir Abdollahinia, Elaheh and Amiryaghoubi, Nazanin and Fathi, Marziyeh and Barar, Jaleh and Omidi, Yadollah (2023) Injectable thermosensitive chitosan/gelatin hydrogel for dental pulp stem cells proliferation and differentiation. BioImpacts, 13 (1). pp. 63-72. ISSN 2228-5660

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Abstract

Injectable thermosensitive chitosan/gelatin hydrogel for dental pulp stem cells proliferation and differentiation Mohammad Samiei Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran Department of Endodontics, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran http://orcid.org/0000-0002-3557-0666 Elaheh Dalir Abdollahinia Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran http://orcid.org/0000-0002-3168-7998 Nazanin Amiryaghoubi Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran http://orcid.org/0000-0002-7237-5595 Marziyeh Fathi Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran http://orcid.org/0000-0001-5710-6365 Jaleh Barar Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran Department of Pharmaceutics, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran http://orcid.org/0000-0002-0105-919X Yadollah Omidi Department of Pharmaceutical Sciences, College of Pharmacy, Nova Southeastern University, Fort Lauderdale, FL 33328, USA http://orcid.org/0000-0003-0067-2475

Introduction: Biocompatible and biodegradable scaffolds based on natural polymers such as gelatin and chitosan (CS) provide suitable microenvironments in dental tissue engineering. In the present study, we report on the synthesis of injectable thermosensitive hydrogel (PNIPAAm-g-CS copolymer/gelatin hybrid hydrogel) for osteogenic differentiation of human dental pulp stem cells (hDPSCs). Methods: The CS-g-PNIPAAm was synthesized using the reaction of carboxyl terminated PNIPAAm with CS, which was then mixed with various amounts of gelatin solution in the presence of genipin as a chemical crosslinker to gain a homogenous solution. The chemical composition and microstructures of the fabricated hydrogels were confirmed by FT-IR and SEM analysis, respectively. To evaluate the mechanical properties (e.g., storage and loss modulus of the gels), the rheological analysis was considered. Calcium deposition and ALP activity of DPSCs were carried out using alizarin red staining and ALP test. While the live/dead assay was performed to study its toxicity, the real-time PCR was conducted to investigate the osteogenic differentiation of hDPSCs cultured on prepared hydrogels. Results: The hydrogels with higher gelatin incorporation showed a slightly looser network compared to the other ones. The hydrogel with less gelatin indicates a rather higher value of G', indicating a higher elasticity due to more crosslinking reaction of amine groups of CS via a covalent bond with genipin. All the hydrogels contained viable cells with negligible dead cells, indicating the high biocompatibility of the prepared hydrogels for hDPSCs. The quantitative results of alizarin red staining displayed a significant rise in calcium deposition in hDPSCs cultured on prepared hydrogels after 21 days. Further, hDPSCs cultured on hydrogel with more gelatin displayed the most ALP activity. The expression of late osteogenic genes such as OCN and BMP-2 were respectively 6 and 4 times higher on the hydrogel with more gelatin than the control group after 21 days. Conclusion: The prepared PNIPAAm-g-CS copolymer/gelatin hybrid hydrogel presented great features (e.g., porous structure, suitable rheological behavior, and improved cell viability), and resulted in osteogenic differentiation necessary for dental tissue engineering.
06 20 2022 01 01 2023 63 72 1 10.34172/crossmark_policy bi.tbzmed.ac.ir false Tabriz University of Medical Sciences Tabriz University of Medical Sciences 2021-06-21 2022-06-15 2022-06-20 10.34172/bi.2022.23904 20230108213057 https://bi.tbzmed.ac.ir/Article/bi-23904 https://bi.tbzmed.ac.ir/PDF/bi-13-63.pdf https://bi.tbzmed.ac.ir/PDF/bi-13-63.pdf https://bi.tbzmed.ac.ir/PDF/bi-13-63.pdf https://bi.tbzmed.ac.ir/PDF/bi-13-63.pdf https://bi.tbzmed.ac.ir/PDF/bi-13-63.pdf Ann Ib Postgrad Med Knowledge of consequences of missing teeth in patients attending prosthetic clinic in UCH Ibadan Dosumu O 12 42 2014 10.1177/0022034517706678 10.1177/154405910208101008 10.1016/j.dental.2011.11.025 10.2174/1874210601307010076 10.1016/j.progpolymsci.2012.09.001 10.1002/adma.200802106 10.7150/thno.46450 10.1016/j.mser.2016.11.001 10.1016/j.ijpharm.2019.01.030 Tissue Eng Regen Med Thermosensitive hydrogels for tissue engineering Kim MS 8 117 2011 10.1016/j.progpolymsci.2004.08.003 10.1039/B713803M 10.1016/j.progpolymsci.2009.05.002 10.1039/C5PY00998G 10.1016/j.carbpol.2005.07.017 10.1016/j.eurpolymj.2012.12.009 10.3389/fbioe.2020.587658 10.1016/j.dental.2019.01.018 10.1007/s00784-019-02906-z 10.1016/j.ijbiomac.2011.06.011 10.1016/j.dental.2020.09.021 10.1002/jbm.a.31431 10.1016/j.biomaterials.2005.05.036 10.1002/btm2.10124 10.1039/C3TB21002B 10.1016/j.joen.2019.03.004 10.1016/j.carbpol.2009.01.016 10.1016/j.joen.2014.12.002 10.1016/j.ijbiomac.2011.04.008 10.1093/nar/29.9.e45 10.1002/mabi.200600142 10.1016/j.ijbiomac.2019.01.122 10.1016/j.ijbiomac.2020.06.138 10.1023/A:1014041003617 10.1039/C9RA08162C 10.1016/j.biomaterials.2004.01.051 10.1016/j.biomaterials.2004.08.006 10.1016/j.biomaterials.2005.05.060 10.1016/j.ab.2004.02.002 10.1371/journal.pone.0020498 10.1039/c5bm00482a 10.1159/000145596 10.1186/s40824-018-0115-0 10.1186/s13287-018-0942-x 10.1089/ten.tec.2011.0140 10.1002/1097-4636(200011)52:2<270::AID-JBM5>3.0.CO;2-1 Chang YL, Stanford CM, Keller JC. Calcium and phosphate supplementation promotes bone cell mineralization: implications for hydroxyapatite (HA)-enhanced bone formation. J Biomed Mater Res 2000.52: 270-8. https://doi.org/???. 10.1007/s00441-014-1938-1 10.18388/abp.2016_1488 10.1186/s13287-015-0162-6 Am J Transl Res Potential mechanisms underlying the Runx2 induced osteogenesis of bone marrow mesenchymal stem cells Xu J 7 2527 2015 10.1089/ten.TEA.2014.0096 10.1016/j.cellsig.2010.10.003 10.1155/2017/7859184 10.1016/j.job.2020.05.004 10.1152/physrev.1989.69.3.990 10.1073/pnas.73.5.1447 10.1088/1748-6041/4/2/025004 10.1016/j.cbi.2015.03.021 10.30491/JABR.2020.105919

Item Type: Article
Subjects: GO for ARCHIVE > Medical Science
Depositing User: Unnamed user with email support@goforarchive.com
Date Deposited: 31 Mar 2023 06:09
Last Modified: 30 Dec 2023 13:26
URI: http://eprints.go4mailburst.com/id/eprint/460

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