STM Article Repository

Pesticides are widely used due to their effectiveness against a wide range of insect pests. Its presence has an impact on aquatic flora and fauna. The current study looks at the acute toxicity of endosulfan to Chironomus stratipennis 4th instar larvae, a common and widespread Indian species. Chironomus stratipennis is an excellent research tool for studying polytene chromosomal band maps and puffing activity. There is a scarcity of information about the biology, band maps, and puffing activity of species found in Maharashtra, particularly in the Nasik region. In Chironomus striatipennis from the Nasik region, the current study looked at normal puffing of fourth instar larvae and puff induction after pesticide exposure in vivo and in vitro. Endosulfan-induced puff was observed in the 13A and 14C regions of the III chromosome arm in vivo, as well as the 14C area of the III chromosomal arm in vitro.