STM Article Repository

Bioluminescence is a natural phenomenon found in living things that emit light without emitting heat.
Bioluminescence can be produced in three ways, namely from special photocytes cells, glands in
tissues, and luminescent bacteria that are symbiotic to the host. Bioluminescence in Loligo sp and
Harpadon nehereus is known to originate from luminescent bacteria which are symbiotic in
luminescent organs. However, information about symbiotic bacteria in other types of luminescence
biota and its potential in Indonesian waters has not been widely explored. This study aims to
determine the potential of luminescence symbiotic bacteria in Loligo sp. and Loligo edulis from Jepara
waters and Harpadon nehereus from the Malacca Strait waters, Indonesia as an anti-bacterial agent
and to prove differences in the luminescence proteins produced by symbiotic bacteria and their hosts.
Sample collection is done by isolating the bacteria from luminescent organs which are then used as
research samples. The study was conducted using a qualitative activity test on pathogenic bacteria
and analysis of SDS-PAGE electrophoresis protein in bacteria and its host. The study found 2 isolates
of luminescent symbiotic bacteria that were potential as antibacterial agents against Multi Drug
resistant (MDR) pathogens of Escherichia coli, with inhibition zones of 32.59 mm and of Enterobacter
sp.5 with inhibition zones of 28.44 mm. Luminescent symbiotic bacteria of L. edulis have the potential
to be antibacterial agent against Vibrio harveyi, E. coli, Staphylococcus aureus, and Bacillus sp.
Analysis of H. nehereus symbiotic bacteria showed gamma hemolytic in blood agar test. Protein
analysis of SDS-PAGE electrophoresis showed that the enzymes found insymbionic bacteria were
different from the enzymes in their host.