STM Article Repository

This paper presents the results of in vitro propagation of Adenosma indianum (Lour.) Merr. The obtained results indicated that shoot regeneration from stem explant achieved on MS basal medium supplemented with 0.5 mg/L BAP after 4 weeks of culture, 46.7% of samples regenerating shoots with an average value of 1.8 shoots per sample. The MS medium containing 0.30 mg/L NAA was suitable for callus induction from leaves and containing 0.50 mg/L BAP and 0.10 mg/L NAA had a highest ability to produce buds from callus, with a ratio of 43.52%, and 3.43 shoots/callus. The multiplication coefficient was greatest on MS medium consist of 0.5 mg/L BAP and 0.3 mg/L IBA, average number of shoots per explant was 10.03 after 6 weeks of culture. The MS medium supplemented with 0.25 mg/L NAA was optimal for rooting (average value of 7.17 roots per shoot). The plantlets were acclimatized and transplanted successfully in the coconut coir pots, the plantlet survival rate was 91.67% after two months of cultivation. The in vitro propagation procedure of A. indianum (Lour.) Merr was developed successfully, shoots can be regenerated directly from axillary bud or callus and in vitro plantlets grow well in greenhouse.