STM Article Repository

The effect of different concentrations of plant growth regulators and their combinations were used to study the direct regenerative response of three sorghum genotypes, CO-S-28, CO-30 and M35-1 using their shoot apex explants. The study revealed the unique potential of TDZ at 1.0 mg/L, in eliciting an efficient shooting response from shoot tip explants irrespective of genotypes. Further, the stimulatory effect of BAP over shoot organogenesis varied with genotypes in relevance to concentration and a combination of BAP and TDZ at 1.0 mg/L each, exhibited a synergistic effect on shoot proliferation. In vitro rhizogenesis studied using the auxins - IAA, IBA and NAA showed that the root formation was highly proliferative at 1.0 mg/L of IBA. Thus, the most elite genotype was identified to be CO-S-28, yielding an efficient regenerative response of 34.6 multiple shoots in MS medium containing 1.0 mg/L of TDZ and a highest number of 14.1 roots in half-strength MS medium supplemented with 1.0 mg/L of IBA mg/L. The fully grown plantlets were transferred to green house with 80% survival rate and no morphogenetic variations were observed among the in vitro grown plants.