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Yang, Xingdong and Li, Qingmei and Kwee, Sharon and Yang, Jifei and Zhang, Qianqian and Hu, Xiaofei and Khamesipour, Faham (2024) An immunochromatographic strip sensor for marbofloxacin residues. PLOS ONE, 19 (3). e0299709. ISSN 1932-6203

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Abstract

Marbofloxacin (MBF) was once widely used as a veterinary drug to control diseases in animals. MBF residues in animal food endanger human health. In the present study, an immunochromatographic strip assay (ICSA) utilizing a competitive principle was developed to rapidly detect MBF in beef samples. The 50% inhibitory concentration (IC50) and the limit of detection (LOD) of the ICSAs were 2.5 ng/mL and 0.5 ng/mL, respectively. The cross-reactivity (CR) of the MBF ICSAs to Ofloxacin (OFL), enrofloxacin (ENR), norfloxacin (NOR), and Ciprofloxacin (CIP) were 60.98%, 32.05%, 22.94%, and 23.58%, respectively. The CR for difloxacin (DIF) and sarafloxacin (SAR) was less than 0.1%. The recovery rates of MBF in spiked beef samples ranged from 82.0% to 90.4%. The intra-assay and interassay coefficients of variation (CVs) were below 10%. In addition, when the same authentic beef samples were detected in a side-by-side comparison between the ICSAs and HPLC‒MS, no statistically significant difference was observed. Therefore, the proposed ICSAs can be a useful tool for monitoring MBF residues in beef samples in a qualitative and quantitative manner.

Item Type: Article
Subjects: GO for ARCHIVE > Biological Science
Depositing User: Unnamed user with email support@goforarchive.com
Date Deposited: 30 Mar 2024 11:50
Last Modified: 30 Mar 2024 11:50
URI: http://eprints.go4mailburst.com/id/eprint/2191

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