STM Article Repository

Acinetobacter baumannii is a major opportunistic nosocomial pathogen. Sodium chloride is one of the elements that is necessary for the survival of some bacterial species. A. baumannii does not show a high salt tolerance, unlike other salt-tolerant bacteria. The purpose of this study is to identify the effect of NaCl on A. baumannii. In this study two isolates of A. baumannii have been used, the local isolate HHR1 and the global isolate ATCC 17904. The isolates were routinely cultured on Nutrient agar and MacConkey agar media under aerobic conditions at 37oC for 24 hours. Firstly, the influence of NaCl on the growth of two isolates of A. baumannii was monitored and the effect of two concentrations of NaCl on Lipopolysaccharide components was determined by SDS-PAGE. SDS-PAGE was done by using the Bio-Rad system according to, using Promega (USA) system. 12 % resolving gel and 4 % stacking gel were prepared and set. The result showed that HHR1 after 20 hr of incubation period reached 0.6 at OD600 in the presence of salt, while the control was continued, whereas, A. baumannii ATCC didn’t exert any defect growth. Moreover, NaCl has greatly affected the LPS components, where only O-antigen has appeared under 1% NaCl in A. baumannii HHR1 and 3% NaCl in A. baumannii ATCC.