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A field experiment was carried out during zaid season of 2021 at crop research farm of SHUATS, Prayagraj to study about the Effect of Spacing and Biofertilizer on growth and yield of Black gram (Vigna mungo L.) The experiment was laid out in randomized block design by keeping three spacing levels, i.e. S - (20 x 10 cm), S2 – (25 x 10 cm) and S3 – (30 x 10 cm) and Biofertilizers i.e. PSB and Rhizobium and which was replicated three. Results revealed that spacing of 30 x 10 cm + Rhizobium , PSB recorded significantly higher in plant height (43.88 cm), number of branches per plant (6.81), number of nodules per plant (25.84), number of pods per plant (37.30), number of seeds per pod (7.51) test weight (37.73 g), grain yield (836 kg/ha) and stover yield (2144 kg/ha) and plant dry weight (6.77 g/plant), crop growth rate results are showed in 20 x 10 cm + Rhizobium + PSB. However, net returns (54550.00 INR/ha) and B:C ratio (2.62) was also obtained with the application of spacing 30 x 10 cm + Rhizobium + PSB. Therefore I concluded that spacing of 30 cm x 10 cm + Rhizobium + PSB was produced more grains (836 kg/ha) and economic effect (2.62).