STM Article Repository

Aims: To determine the frequency of Extended-Spectrum Beta-lactamase producing E. coli strains in cattle farms in Abidjan district (Ivory Coast).

Methodology: A total of 420 bovine faecal samples were collected in five townships in Abidjan district over six (6) month period from April to September 2016. ESBL-producing E. coli strains were isolated on Rapid E. coli 2 medium supplemented with 2 mg / L ceftazidime (antibiotic). The antibiotic resistance profile was evaluated by the diffusion method in agar media and the detection of strains of Extended-Spectrum Beta-lactamase producing E. coli was performed by the double-disk synergy test. Molecular detection of CTX-M, SHV and TEM genes was performed by polymerase chain reaction (PCR).

Results: Out of the 85 strains of E. coli isolated (20,2%), 45 strains were confirmed producing ESBL, a prevalence of 52,9%. The blaCTX-M, blaSHV and blaTEM genes were detected in 15 strains of Extended-Spectrum Beta-lactamase producing E. coli resistant to quinolone with respective frequencies of 73,3%, 66,7% and 100%.

Conclusion: This study showed a high prevalence of Extended-Spectrum Beta-lactamase producing E. coli strains in cattle faeces in the Abidjan district. A good use of antibiotics would be an alternative to overcome the problem of the emergence of Extended-Spectrum Beta-lactamase producing E. coli strains in farms .