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The aim of this experiment was to evaluate the proximate, mineral, vitamin and amino acid composition of Prosopis africana seed oil (PASO). The proximate revealed the presence of moisture (5.62%), crude protein (30.71 %), crude fibre (6.47%), ash (5.08%) and energy (383.26 Kcal/g). Mineral analysis of PASO showed that it contains calcium, potassium, potassium, magnesium, sodium, manganese, zinc, copper and iron at 403.8 mg/100g, 606.1 mg/100g, 142.8 mg/100g, 281.3 mg/100g, 101.7 mg/100g, 38.56 mg/100g, 19.10 mg/100g, 42.28 mg/100g and 14.93 mg/100g respectively. Amino acid such as lysine (4.18 g/100g), histidine (2.31 g/100g), arginine (4.85 g/100g), aspartic acid (8.61 g/100g), threonine (2.80 g/100g), serine (3.11 g/100g), glutamic acid (11.44 g/100g), proline (3.29 g/100g), glycine (3.47 g/100g), alanine (5.22 g/100g), cystine (1.08 g/100g), valine (4.72 g/100g), methionine (1.40 g/100g), isoleucine (3.22 g/100g), leucine (8.31 g/100g), tyrosine (2.40 g/100g), tryptophan (0.90 g/100g) and phenyl alanine (3.85 g/100g) were found to be abundant in PASO. Vitamin analysis of the test material shows that vitamin E had the highest concentration of 11.67 mg/100g followed by vitamin C (8.56 mg/100g), vitamin A (0.55 mg/100g), vitamin D (0.33 mg/100g), vitamin B3 (0.25 mg/100g), vitamin B1 (0.21 mg/100g), vitamin B12 (0.20 mg/100g), vitamin B6 (0.18 mg/100g), vitamin B5 (0.10 mg/100g), vitamin B2 (0.08 mg/100g), vitamin K (0.08 mg/100g) and vitamin B9 (0.06 mg/100g) respectively. It was concluded that PASO is rich in several nutrients that have numerous health benefits including tissue maintenance, coordination of body functions and other biochemical and physiological functions in the body.